Empore™ E4Technology:
An enhanced E3Technology™ for low-cell and single-cell proteomics.
Empore™ E4Technology™ is designed to prepare LCMS-ready samples in an unprecedented manner. The high efficiency and effectiveness along with versatility and low cost make it ideal for processing protein samples for basic research, clinical screening, and large-scale biomarker discovery. The “single vessel” nature of the technology also holds great value for “ultra-low-input” proteomics.
E4Technology™ Workflow
E4Technology™ Results
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Key Features of E4Technology™
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No cell lysis; fixing-and-go samples immediately ready for protein digestion.
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Works with any cell types, such as mammalian cells, bacteria (gram positive and negative), yeast, virus, plant samples (leaf, pollen, seeds), frog embryos, and neural tissues.
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Excellent technical reproducibility; standardiable.
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E4tip XL, E4tip, and E4tip Ultra can accommodate different sample inputs.
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Our E4plate and Empore MiniLab robot are right here for your large sample volumes.
E4Technology™ Applications
Figure 1. Qualitative assessment of E3technology (E3filter) for E. coli proteome analysis
(A-C) Comparison of the number of proteins, peptides, and PSMs between the E3filter, FASP, and SP4-GB approaches. Error bars represent three replicates.
(D-E) Overlapping analyses of proteins and peptides derived from the three methods
Figure 2. Quantitative assessment of E3technology (E3filter) for E. coli proteome analysis.
(A) Coefficient of variation of quantified proteins by the three methods.
(B) Coefficient of variation of quantified peptides.
(C) Percentages of missed cleavages.
(D) Pearson correlation of replicate experiments of E3technology.
Figure 3. Applying E3technology to various sample types.
Mammalian cell (HeLa), tissue (mouse kidney), and body fluid (human saliva) were tested using either E3tip, E3filter, or E3plate. Histograms showed the identification rates of unique proteins and peptide groups. Error bars represent biological replicates.
Resources for E4Technology™
Simple In-Cell Processing Enables Deep Proteome Analysis of Low-Input Caenorhabditis elegans