Empore™ StageTips
The original micro-purification solution for proteomics - trusted by researchers for more than 20 years
Proteomics Optimized
Pre-packed & Ready
High Reproducibility
Built on Empore™ Membrane SPE Technology: The same high-quality Empore™ membrane trusted by proteomics researchers worldwide. Pre-packed in chemically resistant micropipette tips for immediate use - no manual preparation needed. Learn more about SPE technology →
Technical Specifications
Tip Volume
10 µL, 100 µL, 200 µL
Standard Packing
2 layers
Pack Size
96 tips, 384tips
Case Size
960 tips (10 packs)
Tip Material
Polypropylene
Chemical Resistance
Common organic solvents
Shelf Life
Around 3 years at room temperature
Max Layers
Up to 6 layers
Why Choose Empore™ StageTips?
Save time and improve reproducibility with standardized, pre-packed tips
Save Time
Get StageTips pre-packed and avoid manual preparation in the lab. Eliminate the hours spent punching disks and packing tips - focus on your research instead.
Superior Reproducibility
Standardized packing ensures consistent tip-to-tip performance, avoiding the variability encountered during manual preparation. Achieve reliable results across all experiments.
Universal Compatibility
Chemically resistant polypropylene tips work with all organic solvents and acidic/basic conditions needed for protein and peptide desalting and fractionation.
Flexible Options
Mix and match up to 6 layers of different sorbent phases. Available with single sorbents or pre-configured multi-layer combinations for specialized applications.
Cost Effective
Save money by ordering StageTips by the case (960 tips). Standard 200 µL volume with 96 tips per pack provides excellent value for high-throughput labs.
Proven Performance
More than 20 years of trusted use in proteomics labs worldwide. The original StageTip technology that researchers rely on for critical applications.
Empore™ C18 StageTips Operation Steps
Complete peptide cleanup in under 30 minutes with our validated 5-step protocol - from sample preparation to MS-ready peptides
1
Conditioning
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Filter through 50 – 100 µL of methanol without letting all solution pass, keeping the membrane wet. Discard filtrate.
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Repeat conditioning if membrane has gone dry.
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For centrifugation processing we recommend 1,000 – 1,500 x g for all steps.
2
Equilibrating
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Filter through 50 – 100 µL of 0.5% trifluoroacetic acid without letting all solution pass, keeping the membrane wet. Discard filtrate.
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Restart protocol at conditioning if membrane has gone dry.
3
Sample loading
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Put StageTip into a new collection tube and pipette sample into StageTip. Maximum volume of 400 µL. 40 µg maximum recommended peptide mass per C18-HD StageTip.
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Pass all solution through membrane and keep filtrate for troubleshooting problems.
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Optionally, user may pass the sample filtrate through an additional time, which may improve retention.
4
Washing
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Put StageTip into a new collection tube.
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Filter through 50 – 100 µL of 0.5% trifluoroacetic acid.
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Filter through 50 – 100 µL of 0.5% acetic acid.
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Depending on the level of contaminants in the sample, more wash steps may be necessary.
5
Sample eluting
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Put StageTip into a new collection tube for your analyte.
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Filter through 10 – 30 µL of 80% acetonitrile, 0.5% acetic acid, in water.
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Repeat step 5b one to two more times to elute all the analyte.
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Elution volume for high recovery may depend on how many micrograms of sample loaded. At maximum capacity, 100 µL total over a few elutions may be necessary.
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The sample is clean and ready for further concentration followed by mass spectrometry analysis.
Proven Protocols
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips
CDS Analytical continues to manufacture the same, high quality Empore membrane that has been trusted by researchers for their StageTip needs for nearly 20 years. Our standardized production ensures consistent performance across all batches.
"Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips"
Rappsilber J., Mann M. & Ishihama Y. - Nature Protocols (2007)
Stagetips Selection Guide
Choose the optimal size for your sample volume and application
10 µLm vs 200 µL Disk Selection Guide
10 µL
Micro-Scale Analysis
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Sample volume: 0.1-10 µL
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Ultra-sensitive proteomics
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Single-cell applications
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96 tips per pack
200 µL
Standard Processing
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Sample volume: 10-200 µL
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Routine proteomics workflows
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Peptide fractionation
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High-throughput processing
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96 tips per pack
Empore™ Stagetips Performance
Quantitative results demonstrating superior reproducibility
Applications
Optimized for proteomics and peptide analysis workflows
Proteomics Sample Preparation
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Peptide desalting before MS
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Buffer exchange
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Removal of detergents
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Concentrate Sample
Peptide Fractionation
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SCX fractionation
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SAX fractionation
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High pH RP fractionation
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2D LC preparation
Biomarker Discovery
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Plasma/serum peptide profiling
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Urine peptidome analysis
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CSF protein digests
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Tissue extract cleanup
Metabolomics
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Small molecule cleanup
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Lipid extraction
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Metabolite fractionation
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Sample storage
Quantitative Proteomics
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TMT/iTRAQ sample prep
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SILAC workflow support
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Label-free quantification
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DIA sample preparation
Products Selection Guide
Choose the right sorbent chemistry for your application
Resources for StageTips
Download technical documents and application notes
Using the Empore™ C18 StageTips for High pH Peptide Fractionation in Global Proteomics
Evaluation of the Sample Loading Amount for Four Types of StageTips on Total Number of Protein Identifications in Proteomics
Evaluation of Different Empore™ StageTips for Proteomics Applications