First quantitative DIA-MS proteomic analysis of barramundi (Lates calcarifer) tissues comparing two commercial diets differing in protein source. Empore SDB-RPS StageTips are used for peptide desalting prior to nanoLC-MS/MS analysis, revealing diet-driven proteomic changes across brain, liver, and intestine linked to ferroptosis, iron metabolism, and digestive enzyme regulation.

Develops Deltafluorine, a benzyl fluoride-based covalent inhibitor that selectively modifies glutamate p.E88 in the KRAS chaperone PDEδ, impairing MAPK and Akt-mTOR signaling. Empore C18 disks are used for StageTip desalting of Glu-C digested peptides in LC-MS/MS analysis of covalent adducts, confirming selective modification site at p.E88.

Develops orally stable FAHFA bioisosteres (12-TAASA, 12-HDTZSA) that reduce gut-to-liver lipid flux and ameliorate diet-induced liver steatosis. Proteomic target identification uses a SISPROT spin-tip device built with Empore C18 disk plugs to profile biotinylated 12-TAASA pull-down partners from intestinal mucosal lysates, identifying CD44 as a key epithelial lipid-uptake regulator.

Develops Rapid HAMMOC, a streamlined TiO2-based phosphopeptide enrichment workflow using Empore C8, SDB, and SAX disk membranes to construct StageTips—including a novel tandem SAX-SDB StageTip for direct phosphopeptide desalting—enabling identification of ~8,000 class I phosphosites from as little as 0.5 μg cell digest input.

Uses differential ultracentrifugation combined with quantitative proteomics (dynamic organellar maps) to show that structurally diverse NLRP3 activators uniformly displace the endocytic AP2 complex from the plasma membrane, identifying this disruption as a common surveillance checkpoint linking impaired GPCR signaling to inflammasome activation and pyroptosis.

Compares passive samplers (POCIS and Chemcatcher) with grab sampling techniques (SBSE and SPE) for pesticide monitoring in Australian surface waters, screening 230 pesticides across three sites with distinct land uses and evaluating suitability for citizen science applications.

Investigates proteome dynamics across three fermentation stages in sequential cultures of S. bacillaris FRI751 and S. cerevisiae EC1118, revealing that S. bacillaris significantly modulates S. cerevisiae's proteome and accelerates reduction of cell wall enzymes relevant to wine quality.

Uses cell surface proximity biotinylation coupled with mass spectrometry to map the PD-L1 interactome in melanoma cells, identifying tetraspanin TSPAN4 as a novel negative regulator that promotes PD-L1 degradation and restricts its lateral mobility at the plasma membrane.

Combines RNA-seq and Ribo-seq across ten developmental stages of maize anthers to map translational dynamics, identifying functional ORFs in 3′ UTRs linked to male sterility — including the APV1 locus — and revealing reduced photosynthetic activity in anther tissue.

Analyzes associations between urinary genistein, daidzein, and equol concentrations and semen parameters in 157 Japanese men seeking fertility treatment, finding negative associations between isoflavone levels and sperm count and concentration measured by LC-MS/MS.