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Expert application notes, method guides, and technical insights powering your SPE and proteomics research.
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How to Desalt Peptides for LC-MS/MS Analysis Using C18 StageTips: A Step-by-Step Protocol
A complete, practical guide to peptide desalting with Empore™ C18 StageTips — from sample acidification through elution — with troubleshooting tips and best practices for maximizing peptide recovery. Authors: CDS Empore™ Technical Team Introduction Peptide desalting is one of the most critical — and most underappreciated — steps in any bottom-up proteomics workflow. After enzymatic digestion, your peptide sample typically contains salts, buffers, urea, detergents, or other re
6 min read


EPA Method 8270E: Extract 125 Semi-Volatile Organic Compounds from Water Using the Empore™ 8270 Disk
Authors: Xiaohui Zhang, Guotao Lu, Michael Apsokardu A validated EPA Method 8270E workflow using the CDS Empore™ 8270 Disk extracts 125 semi-volatile organic compounds (SVOCs) from 250 mL water samples spiked at 20 µg/L. Most compounds achieved recovery greater than 75% with RSD below 15% (n=3), making the workflow a versatile, regulator-compliant option for wastewater detection laboratories operating under EPA 8270E and SW-846 Method 3535. Study at a Glance Introduction: The
8 min read
Life Science


THC-COOH Urine Extraction Using the Empore™ Membrane C18 Cartridge
Authors: Xiaohui Zhang, Guotao Lu, Michael Apsokardu Study summary: A validated solid-phase extraction (SPE) workflow using the Empore™ 3 mL C18 membrane cartridge (Model 4215SD) extracts the THC metabolite 11-nor-9-carboxy-Δ⁹-tetrahydrocannabinol (THC-COOH) from urine for GC-MS quantitation. After alkaline hydrolysis (10 M KOH, 60°C, 15 min) and pH adjustment to 4.0–4.5, urine is loaded onto the cartridge by gravity, washed with 50:50 methanol/water, and eluted with 75:25 n-
9 min read
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