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Expert application notes, method guides, and technical insights powering your SPE and proteomics research.
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How to Desalt Peptides for LC-MS/MS Analysis Using C18 StageTips: A Step-by-Step Protocol
A complete, practical guide to peptide desalting with Empore™ C18 StageTips — from sample acidification through elution — with troubleshooting tips and best practices for maximizing peptide recovery. Authors: CDS Empore™ Technical Team Introduction Peptide desalting is one of the most critical — and most underappreciated — steps in any bottom-up proteomics workflow. After enzymatic digestion, your peptide sample typically contains salts, buffers, urea, detergents, or other re
6 min read
EPA Method 8270E: Extract 125 Semi-Volatile Organic Compounds from Water Using the Empore™ 8270 Disk
Authors: Xiaohui Zhang, Guotao Lu, Michael Apsokardu A validated EPA Method 8270E workflow using the CDS Empore™ 8270 Disk extracts 125 semi-volatile organic compounds (SVOCs) from 250 mL water samples spiked at 20 µg/L. Most compounds achieved recovery greater than 75% with RSD below 15% (n=3), making the workflow a versatile, regulator-compliant option for wastewater detection laboratories operating under EPA 8270E and SW-846 Method 3535. Study at a Glance Introduction: The
8 min read
EPA Method 1613B: Dioxin & Furan Analysis in Industrial Wastewater Using the Empore™ C18 SPE Disk
Authors: Guotao Lu Study summary: A validated EPA Method 1613 Rev. B workflow using the Empore™ C18 SPE Disk extracts 16 toxic 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) from 1 L industrial wastewater samples at pH 2. Across four matrices — POTW final effluent, petroleum refinery API separator effluent, refinery final treatment effluent, and river water — all 16 native compounds fell within the EPA-required 75%–125% acceptance wind
11 min read
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