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Expert application notes, method guides, and technical insights powering your SPE and proteomics research.
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How to Desalt Peptides for LC-MS/MS Analysis Using C18 StageTips: A Step-by-Step Protocol
A complete, practical guide to peptide desalting with Empore™ C18 StageTips — from sample acidification through elution — with troubleshooting tips and best practices for maximizing peptide recovery. Authors: CDS Empore™ Technical Team Introduction Peptide desalting is one of the most critical — and most underappreciated — steps in any bottom-up proteomics workflow. After enzymatic digestion, your peptide sample typically contains salts, buffers, urea, detergents, or other re
6 min read
EPA Method 8270E: Extract 125 Semi-Volatile Organic Compounds from Water Using the Empore™ 8270 Disk
Authors: Xiaohui Zhang, Guotao Lu, Michael Apsokardu A validated EPA Method 8270E workflow using the CDS Empore™ 8270 Disk extracts 125 semi-volatile organic compounds (SVOCs) from 250 mL water samples spiked at 20 µg/L. Most compounds achieved recovery greater than 75% with RSD below 15% (n=3), making the workflow a versatile, regulator-compliant option for wastewater detection laboratories operating under EPA 8270E and SW-846 Method 3535. Study at a Glance Introduction: The
8 min read
How to Extract Pesticides and Flame Retardants from Drinking Water: A Complete EPA Method 527.1 Protocol
Authors: Xiaohui Zhang, Michael Apsokardu Study summary: A validated EPA Method 527.1 workflow using the Empore™ SDB-XC SPE Disk with the EZ-Trace SPE Workstation extracts 26 pesticides and flame retardants (including pyrethroids, organophosphates, and polybrominated diphenyl ethers) from 1 L drinking water samples. With 1.0 and 2.0 µg/L spike levels (n=4), 92% of analytes fell within EPA's 70-130% acceptable recovery range with average recovery of 102.7% (RSD 15.8%) and 102.
7 min read
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